Thermo-Responsive Stationary Phases for High Performance Liquid Chromatography (HPLC)
Maria Magdalena Titirici, Farnoosh Roohi, Max-Planck Institute for Colloids and Interfaces, Research Campus Golm
In classical liquid chromatography, proteins are generally separated by employing RP-18 columns in the presence of acetonitrile or other organic solvents at low pH buffers as mobile phase. However, the presence of organic solvents often leads to the total denaturation of proteins, while acidic en...