Ellman's assay for in-solution quantification of sulfhydryl groups
Rucha Bhat, Gregory Grover, Contraline Inc.
Ellman introduced 5,5'-dithio-bis-(2-nitrobenzoic acid), also known as DTNB, as a compound used for quantitating free sulfhydryl groups in solution. A solution of this compound produces a yellow-colored product when it reacts with sulfhydryl groups. This yellow-colored product can be measured usi...