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Contraline Inc.
Ellman's assay for in-solution quantification of sulfhydryl groups
Rucha Bhat, Gregory Grover, Contraline Inc.
Ellman introduced 5,5'-dithio-bis-(2-nitrobenzoic acid), also known as DTNB, as a compound used for quantitating free sulfhydryl groups in solution. A solution of this compound produces a yellow-colored product when it reacts with sulfhydryl groups. This yellow-colored product can be measured u...