Analytik NEWS
Online Laboratory Magazine
03/29/2024

02/06/2009

Thermo-Responsive Stationary Phases for High Performance Liquid Chromatography (HPLC)

Maria Magdalena Titirici , Farnoosh Roohi , Max-Planck Institute for Colloids and Interfaces, Research Campus Golm


In classical liquid chromatography, proteins are generally separated by employing RP-18 columns in the presence of acetonitrile or other organic solvents at low pH buffers as mobile phase. However, the presence of organic solvents often leads to the total denaturation of proteins, while acidic environments are often destructive to the activity of many enzymes. One possible way to prevent the denaturation of proteins in liquid chromatography is to use purely aqueous mobile phases. However, RP columns do not function well under such conditions.

Recently a novel method has been developed by Kanazawa et al, in which the stationary phase changes its surface properties from hydrophilic to hydrophobic as a response to temperature. The main advantage of these temperature sensitive stationary phases is that they can separate mixtures of biomolecules (large peptides, proteins) in a pure aqueous environment under isocratic conditions. Thus, the harsh conditions normally associated with protein separation and denaturation by employing RP-18 columns in the presence of organic solvents gradients can then be avoided.


» Read article

You have disabled cookies in your browser.


To be able to use this form you have to enable cookies. For more information please have a look at our privacy policy.

If you are not sure how to do this or if you do not have admin privileges, please contact us by email at or by phone at +49 6154 6246 860.

If you did enable cookies, please click the "Submit" button below.