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Thema: Re: Quantifizierung von underivatisierten Aminosäuren mittels LC-MS

Autor(in): Einar Pontén am 06. Januar 2003 um 17:11:28

Antwort auf: Quantifizierung von underivatisierten Aminosäuren mittels LC-MS eingetragen von Ivana am 10. Dezember 2002 um 16:35:51

An option for zwitterionic compounds like amino acids or other polar compounds is to use Hydrophilic Interaction Chromatography (HILIC).

In a typical HILIC set-up an initial high content of acetonitrile is used (60-80% v/v) and if a gradient is run the content of water or buffer is increased.

The ZIC™-HILIC column has permanently bonded zwitterionic functional groups and is suited for HILIC separations. It will retain basic and very hydrophilic amino acids most. For more information, please see http://www.sequant.com

A ref for separation of underivatised amino acids.
Chiral Separations of Polar Compounds by Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection.
Risley, Donald S.; Strege, Mark A.
Analytical Chemistry (2000), 72(8), 1736-1739.